Multiplex PCR for identifying mycobacterial isolates.
نویسندگان
چکیده
منابع مشابه
Multiplex PCR for identifying mycobacterial isolates.
AIMS To develop a multiplex polymerase chain reaction (PCR) method to facilitate identification of mycobacterial isolates. METHODS Type strains of 14 species of mycobacteria and 56 clinical isolates were lysed by boiling in TE Triton. The lysate (5 microliters) was used directly in a PCR reaction incorporating three pairs of PCR primers expected to amplify fragments from the genome of (a) all...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1995
ISSN: 0021-9746
DOI: 10.1136/jcp.48.3.203